Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... from
Caveolin-1 cDNA are GCAAGTGTACGACGCGCAC
and AACCAGAAGGGACACACA, respectively.
As negative controls (scrambled shRNAs), shRNAcontrol
1
(TAGCGACTAAACACATCAA), shRNAcontrol
2
(TATA
GCGACTAAACACATCAA) and ... rafts are microdomains of the plasma
membrane containing a high proportion of sphingo-
lipids and cholesterol, assembled in the Golgi apparatus
and subsequently deli...
... (i.e. the
b-hairpin loop and part of the adjoining b-sheet) and
residues in the loop connecting b-strand and a- helix and
contiguous residues on the a- helix and the last part of
b-strand 3Õ [35]. ... membrane. The way this contact
takes place and the molecular features of the protein
involved are yet to be deciphered. The lack of information
about...
... 2815
(5¢-CCGAGCTCGAGACGTGAAGCGACAATCTCGC
AATCT-3¢) containing an XhoI (Promega) site upstream of
the start codon and an antisense primer (5¢-CAGCCAA
GCTTCTCACTCTTTGATGAAATGCATCT-3¢) con-
taining a HindIII ... Takashi Isobe
1
, Takeshi Arakawa
2
,
Yasunobu Matsumoto
3
and Naotoshi Tsuji
1
1
Laboratory of Parasitic Diseases, National Institute of Animal Health, National Agricult...
... considered as
follows. The role and importance of the aspartate in the
catalytic triad is not fully understood because several serine
proteases do not have an aspartate as the catalytic apparatus.
However, ... dependence; the acidic rim is at pK
a
¼ 6.5 and the
Fig. 1. Stick models of the reactive site in
bovine trypsin and API. The catalytic triad
residue...
... beta- and
gamma-catenins in their cytoplamic tails. As with tight
junctions, adherens junctions are linked to the actin
cytoskeleton via the binding of beta- and gamma-cate-
nin to alpha-catenin ... in ammatory
responses [85]. In addition, the slight edema of astro-
cytes helps maintain the water balance in the brain,
but pathologic cytotoxic edema is a main cause...
... the
identifiable states of the folding pathway and relate to
the amount of solvent-exposed surface area in each of
these states (see Materials and methods). This can be
done for all five states in the ... study, it was ascertained that the first
reaction phase was fast and concentration dependent,
showing that the intermediate was readily populated
and dimeric. The...
... cellular ceramide are regulated by the
de novo pathway and the recycling pathway. The former
relates to the synthesis of ceramide through the conden-
sation of palmitate and serine in a series of ... mutations.
Acknowledgements
This research was supported in part by the Intramural
Research Program of the National Institute on Aging,
National Institutes of Hea...
... organization
Roles of the Las17p (yeast WASP)-binding domain and a novel
C-terminal actin-binding domain
Thirumaran Thanabalu
1,2
, Rajamuthiah Rajmohan
2
, Lei Meng
2
, Gang Ren
4,5
, Parimala R. Vajjhala
4
and ... and immunoblotted with an anti-actin mAb (left). Equivalent
amounts of purified yeast actin were used in each binding assay and an amount representing 10% of t...
... circumscription of
Saccharomyces, Kluyveromyces and other members of
the Saccharomycetaceae, and the proposal of the new
genera Lachancea, Nakaseomyces, Naumovia, Vanderw-
altozyma and Zygotorulaspora. FEMS ... due to amino acid biosynthesis. Much of
the generation of NADH during amino acid biosyn-
thesis takes place in the mitochondria. Because of the
block i...