Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc

... determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory V. Rajaram1*, B. S. Bhavani3*, ... structure of E53QbsSHMT and its complexes V. Rajaram et al.4160 FEBS Journal 274 (2007) 4148–4160 ª 2007 The Authors Journal compilation ª 2007 FEBS Structure determination and biochemical studies on ... the positioncorresponding to the second conformation of thecarboxylate group (Fig. 2A,B). The hydrogen bondingbetween Q53 and the second conformation of carbo-xylate of Gly found in E53QbsSHMT-Gly...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... histone acetylation and deacetyla-tion, and transient recruitment of remodellers and transcription factors.A cycle of transcription commences with the recruit-ment of transcription factors and ... assem-ble complexes containing repressive HMT and DNMTproteins that act in a concerted fashion to maintainboth DNA methylation and H3-K9 methylation [202].However, CG islands usually exist as constitutivelyactive ... (fraction P). These studies foundthat fraction S1 was predominantly mono-nucleosomal and was highly enriched in transcribed genes, but wasdepleted of inactive genes and histone H1; fraction S2contained...

Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx

... Sa2–3¢-GMP and RNase Sa2–2¢-GMP seems tobe in the conformation of the Arg34 side chain, whichappears to depend on whether the mononucleotide isin the syn-conformation or anti-conformation. InRNase ... can be a proton donor forthe leaving O5¢ RNA strand.Taking into consideration the conformation of botharginine side chains in the RNase Sa2–mononucleotidestructures, Arg67 and Arg71 might ... replaces one of the phosphate oxygens in exo-2¢,3¢-GCPT, is dark green. Hydrogen bonds between themononucleotide and RNase Sa2 are shown as dashed lines.Structures of RNase Sa2–mononucleotide complexes...

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

... compilation ª 2008 FEBSevolution and herbivore defense in Brassicaceae. PlantMol Biol 42, 93–113.5Lo¨nnerdal B & Janson JC (1973) Studies on myrosinas-es. II. Puriﬁcation and characterization ... weoptimized the conditions for E. coli cell-free productionof At3g16450.1 with regard to reaction temperature,duration of incubation, and expression vector. For com-parison purposes, [U-13C,U-15N]-labeled ... & Sander C (1983) Dictionary of proteinsecondary structure – pattern-recognition of hydrogen-bonded and geometrical features. Biopolymers 22, 2577–2637.20 Holm L, Ouzounis C, Sander C,...

Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx

... inprotein processing or degradation under different conditions and in different stages of plant development suggests somefunctional specialization. Based on the recent findings on thediversity of A1 family ... protein-processingenzymes responsible for the conversion because, in terms ofprimary structure organization, plant APs precursors are, ingeneral, very similar.Like for monocotyledonous plants, AP expression ... atypical and their localiza-tions are unexpected.New questions concerning functional significance and specialization of this multigene family of proteases are nowstarting to be addressed and will...

Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... three-dimensional structures ofabout half of the known neuronal specific a-conotoxins havenow been determined and have a consensus fold containing ahelical region braced by two conserved disulfide bonds.These ... structuralinformation and the insights into structure- activity relation-ships of a-conotoxins is presented in this review.Structural features of a-conotoxinsThe three-dimensional structures of a-conotoxins ... predefined structure of this tail region is not present insolution and the three-dimensional structures indicate thatthis region is disordered. However, a particular conforma-tion may be present upon...

Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf

... fusion peptide on its structure, organization and membrane interactions. Biochim. Biophys. Ac ta 1612, 41–51.21. Tamm, L.K. & Tatulian, S.A. (1993) Orientation of functional and nonfunctional ... membrane.Effects of pH on the secondary structure and membraneinteraction of IFP–wtBased o n studies on the molecular clustering of EnvA fusionprotein of ASLV i n response to the receptor binding and low ... activity of IFP–wt and its relationshipto the secondary structure Lipid m ixing measurements on the internal and N-terminalfusion peptides indicate that the IFP has higher fusionactivity than...

Tài liệu Báo cáo khoa học: Structure of peptidase T from Salmonella typhimurium doc

... similar conclusionbased on extensive sequence comparisons between and within the CPA and CG2 families was recently reported [29].In addition, one of the ligands for t he more weakly boundzinc ion ... hexamericLAP contains a second, N-terminal, domain involved insubunit contacts. CG2, on the other hand, is dimeric and has a 110 amino-acid insertion that forms a second domainthat is responsible ... a ligand complex structure has been reported only for APP. The position ofthis ligand and comparison of the zinc ions and thebinding of am astatin t o L AP suggest the location o f t he S1subsite....

Tài liệu Báo cáo khoa học: "Structure-Sharing in Lexical Representation" docx

... taking consultant as the head, and using the information on the lexical frame CONSULTANT- COMMON (which is inherited from the COMMON- NOUN class) that it requires a determiner as its only complement ... information once thought to be clearly the responsi- bility of phrase structure rules is in fact more simply represented as lexical information, once one has the power of a highly structured lexicon ... elimina- tion of most common and proper nouns from the lexicon, one gains not 0nly considerable space savings, but a sharp reduction in the seed for additions to the lexicon by salve users as...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... unlabeleddopamine or serotonin, respectively. The ﬁnal concentrationof dopamine and serotonin ranged from 0–10 lm. For singleVmaxdeterminations, only one concentration (5 lm)of[3H]-dopamine ... the0.1–2.5 mm DA concentration range, and a concentrationof 0.5 mm was used for further survival studies. HEK-MSR-293 cells were transfected in a solution with a ﬁxedconcentration of vector DNA ... aggregation of a-syn, and thereby induces cytotoxicity [16,17]. We hypothe-sized that expression of p25a in dopaminergic neurons,in addition to its putative effects on a-syn aggregation,may...

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