... BMP -R-Smad ortholog from S. mansoni
FEBS Journal 274 (2007) 4075–4093 ª 2007 The Authors Journal compilation ª 2007 FEBS 4093
Identification and characterization of an R-Smad ortholog
(SmSmad1B) from ... Adamson RE et al.
(2003) Transcriptome analysis of the acoelomate human
parasite Schistosoma mansoni. Nat Genet 35, 148–157.
SmSmad1B, a BMP -R-Smad ortholog...
... expression of T-bet has been
seen within a wide variety of tissues and cells in the
Ginbuna crucian carp [27] and, in mammals, in the
lung tissue of mouse and spleen and thymus of human
and mouse ... [44].
The gene organizations of zebrafish t-bet, stat6 and
foxp3 were also determined and found to be very simi-
lar to their human homologues. Both the human and
zebrafish...
... peptidases
of
Bacillus amyloliquefaciens
Hoang Ha Chu, Viet Hoang*, Peter Kreutzmann², Brigitte Hofemeister, Michael Melzer
and JuÈ rgen Hofemeister
Institute of Plant Genetics and Crop Plant Research ... characters
of their processing apparatus with respect to the presence
and specialization of Sip protein homo logous.
ACKNOWLEDGEMENTS
We thank Susanne Ko
È
nig and Christian...
... Almeras Y, Santrot M,
Glansdorff N & Sakanyan V (2000) Characterization
and kinetic mechanism of mono- and bifunctional
ornithine acetyltransferases from thermophilic micro-
organisms. Eur ... independent
experiments and vertical bars are SD. (B) Immunoblot analysis of
DRIP-1 in the total soluble proteins (20 lg per lane) isolated from
plants before (0 day) and after 1,...
... sialidasetypeX,protease
enzymes and molecular mass standards were purchased
from Sigma.
Preparation of crab sera
Freshwater field crabs, Paratelphusa jacquemontii were
collected from the local wetlands of Kanyakumari ... Sarris and Palade [30] and Schauer [6]. A
solution of 750 lL of glycoprotein (5 mgÆmL
)1
) was added
to 250 lLof0.04
M
of NaOH, vortexed, incubated on ice...
... clones of the
pCRÒII TOPO (Invitrogen) and pET-24a(+) (Novagen)
vectors carrying the inserts. T7 and Sp6 primers were used
for both sense and antisense strands. The BigDye termina-
tor method from ... simultaneous expression
of the 14-kDa phosphohistidine phosphatase and an
endosomal protein may be of interest in the light of the
recently described histidine phosphorylatio...
... 1899
absorption maxima at 420 nm (c band), 530 nm (b band)
and 557 nm (a band) are characteristic of cytochrome b.
Heme was extracted from the protein with acidic acetone
and the pyridine hemochrome spectrum ... gel (lane B2). This behavior is
typical of integral membrane proteins. The polypeptide with an
apparent molecular mass of 53 kDa appears as a double band in
unboiled sam...
... coimmu-
noprecipitatedwithGroELfrom1 5and3 0 lg of the cell lysate without
(lanes 1 and 3) or in the presence of ATP (lanes 2 and 4) was detected
with anti-UP12 Ig. Samples (3 and 6 lg of total proteins) of the cell
extract ... Effect of addition of 4 m
M
DNP
on cell growth (h) and expression of UP12
(j). (B) Monitoring of cell growth (s )and
amount of UP12 (d) befor...
... left panels), anti-RIa and TRITC secondary antibody (red, upper middle panel) and anti-
RIIa and TRITC secondary antibody (red, lower middle panel). Right upper and lower panels show merges of Cb2 ... polyclonal antibodies (anti-PKAacat, anti-
PKAbcat and anti-PKAccat) and one monoclonal
antibody (anti-Cmono) were immunoreactive to two
protein bands of 40 and 47 kDa, respect...
... dissociation of the complex was
very fast and the reaction between ligand and analyte
equilibrated very rapidly.
As the association and dissociation phases in the inter-
actions of madanin 1 and 2 with ... inhibit activation of factors
V and VIII by thrombin and then prevent these cofactors
from forming prothrombinase and tenase complexes. On
the other hand, as shown in...