... selection of recombinants. The sitesincorporated were HaeIII, NcoI and BamHI (Table 1). ThePCR mix contained 200 ng of each primer, 20 ng of the tem-plate, 200 lm of each dNTP and 5 units of Taq DNA ... Department of Biotechnology(DBT), Department of science and technology (DST),Council of Scientific and Industrial research (CSIR) and senior research fellowship from CSIR, Govern-ment of India.References1 ... A rapid and sensitive methodfor the quantitation of microgram quantities of proteinutilizing the principle of protein–dye binding. AnalBiochem 72, 24 8–2 54.54 Oesper P & Meyerhof O (1950)...