... been identified as insoluble proteins in
many diseased tissues including amyloid fibrils [7,8].
We are investigating the role of amino acid side chain
oxidation in amyloid assemblies by comparing ... entropy in
binding and folding: general methods and application to
helix formation. Proteins 20, 68–84.
12 Kay MS & Baldwin RL (1996) Packing interactions in
the apomyglobin folding int...
... glucan
synthesis.
Abbreviations
DP, degree of polymerization; GBd, glucan-binding domain; GS, glucan-binding domain-deficient glucosyltransferase-I; GSd, glucansucrase
domain; GSGB, glucosyltransferase-I containing ... are described in Doc. S1.
13
C-NMR analysis
To inhibit base-catalyzed reactions, the insoluble glucan
was dissolved at 60 mgÆ mL
)1
in 0.5 m NaOD in D
2
O con-
taining 3....
... former effect being efficiently increased by a cathepsin B ⁄ cathepsin D
pretreatment. The data show a novel processing pathway for internalized
ETA, involving cathepsins B and D, resulting in the production ... [22].
The antibody against KX
5
KDEL also binds to undefined endosomal
proteins distinct from ETA, both in control and in toxin-injected rats,
whose levels have been shown to...
... hóa học b ng phổ hồng ngoại FTIR. Mẫu b t gỉ thép và gỉ thép đã được xử lý b ng chất biến
đổi gỉ B- 05 trộn với b t KBr và nén thành viên với tỷ lệ 2%, được phân tích FTIR trong dải b ớc sóng ... Trong khi đó, trên b mặt gỉ thép đã
được xử lý b ng chất B- 05 có cấu trúc mịn, đồng nhất, không b xốp rỗng (Hình 1 .b) .
Như vậy b ớc đầu có thể thấy rằng sau khi được xử lý gỉ b mặt...
... proteins by
western blotting analysis. b- Actin was used to normalize equal protein loading; Blot images represent one of three independent experiments.
(C) MTP was determined using a JC-1 probe. ... osteoarthritic joints was increased com-
pared with that in control joints. Changes in TRAIL
and ubiquitin levels may be linked to the progression
of in ammation and may be detected in th...
... were obtained in ChIP DNA, indicating that
the binding of putative CERK-PPRE to PPARb is
specific. Furthermore, the interaction between PPARb
and CERK was confirmed by an EMSA using biotin-
labeled ... of PPARb, which
enhanced cell survival or inhibited cell death induced
by serum starvation.
Next, we investigated the participation of Cer in
cell survival promoted by PPARb, by examining...
... Reser-
voir Counting for application in online Local-
ity Sensitive Hashing. This technique allows
for significant savings in the streaming setting,
allowing for maintaining a larger number of
signatures, ... function, this may be justified in greater
overall fidelity from the resultant increase in b.
1
A related point was made by Li et al. (2008) when dis-
cussing stable random projec...
... treatment, cells were incubated in medium
containing 50 lgÆmL
)1
neutral red at 37 °C for 3 h, then
fixed with 1% formaldehyde containing 1% CaCl
2
for
1 min. The dye incorporated into viable cells ... apoptosis < /b> in F9 cells using
SB202190, a potent inhibitor of p38. As shown in Fig. 7A,
although hydrogen peroxide-treatment induced apoptosis < /b> in
F9 cells as describe...
... curcumin with microtubule inhibitors for tub-
ulin binding. (A) Colchicine and podophyllotoxin weakly inhibited
binding of curcumin to tubulin. Data represent mean ± SEM
(n ¼ 4). (B) Binding of ... 30 min incubation at 25 °C by exciting
the samples at 425 nm.
Binding of curcumin to preformed tubulin–
colchicine or tubulin–podophyllotoxin complex
Tubulin (3 l m) was incubated in the absence...