Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... family proteins FEBS Journal 276 (2009) 4130–4141 ª 2009 The Authors Journal compilation ª 2009 FEBS 4141 Molecular cloning and characterization of soybean protein disulfide isomerase family proteins ... (2008) A novelplant protein disulfide isomerase family homologous toanimal P5 – molecular cloning and characterization as afunctional protein for folding of soybean seed-storage proteins. FEBS J ... 2009)doi:10.1111/j.1742-4658.2009.07123.x Protein disulfide isomerase (PDI) and other PDI family proteins are mem-bers of the thioredoxin superfamily and are thought to play important rolesin disulfide bond formation and isomerization...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... expression of T-bet has beenseen within a wide variety of tissues and cells in theGinbuna crucian carp [27] and, in mammals, in thelung tissue of mouse and spleen and thymus of human and mouse ... the expression of these genes within humans and mice, of T-betwithin Ginbuna crucian carp [27] and STAT6 inmandarin fish [28] have shown similar widespreadexpression. In the case of STAT6, highest ... necessary for STAT protein activity [39].Foxp3 belongs to the fork-head box (FOX) family of proteins [42], a family of transcription factors thatare both transcriptional repressors and activators....
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... spe-cificity [31]. The L3 loop of SmSmad1B groups this protein with other BMP-related R-Smads. The presence of a histidine and an aspartate at positions 340 and 343within the L3 loop of SmSmad1B (Fig. 1A) ... schisto-somes, and also confirm the similarities in the protein interaction properties of SmSmad1 and SmSmad1B asdescribed above. However, in the presence of SmSmad4 and the active mutant form of TbRI, ... constitutively active) on the interaction of SmSmad2 and SmSmad4 with SmGCN5, non-S protein- tagged [35S]methionine-labeled SmSmad2 and SmSmad4(5 lL each), individually and in the presence of the...
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Tài liệu Báo cáo khoa học: Purification and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc
... dithiothreitol(DTT) and 20% (v ⁄ v) glycerol, and 20% saturation of (NH4)2SO4. The enzyme was eluted with a linear gradient of 30–0% saturation of (NH4)2SO4in buffer A. Active fractions ... (5734 Da), cyto-chrome c (12361 Da), myoglobin (16952 and 8476 Da) and ubiquitin (8566 Da; all Sigma) as standard proteins. Western blotting Proteins from SDS ⁄ PAGE were transferred to polyvinyl-dene ... plants, acting as mediators of osmoticadjustment, stabilizers of subcellular structures, and scavengers of active oxygen radicals [4]. The mecha-nisms of proline, mannitol, and glycine betaine accu-mulation...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt
... intenseband of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature(Fig. 1, lane 1).However, in the absence of boiling (Fig. 1A, lanes 2 and 4) high molecular ... structural proteins during theassembly of the head of bacteriophage T4. Nature 227, 680–685.28. Blum, H., Beier, H. & Gross, H.J. (1987) Improved silver staining of plant proteins, RNA and DNA ... partialspectroscopic and redox characterization of the NrfHsubunit is also presented. Up to now, there has been noinformation of this kind on any NrfH protein. Materials and methods Protein purificationccNiR...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... erythro-cytes.De-O-acetylated preparation of glycoproteins. De-O-acetylation of glycoproteins was performed following theprocedures of Sarris and Palade [30] and Schauer [6]. Asolution of 750 lL of glycoprotein (5 mgÆmL)1) ... inhibition (HAI) assays with avariety of sialoglycoproteins (Table 5) showed BSM as themost potent inhibitor of the purified lectin with an HAI of 524 288. The HAI of the sialoglycoproteins can be gradedas ... As a control,glycoproteins were treated similarly without sialidase. TheHAI assay was performed with purified lectins for treated and untreated glycoproteins against 1.5% of horse erythro-cytes.De-O-acetylated...
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Tài liệu Báo cáo khoa học: Purification and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulfide reductase from methanogenic archaea pdf
... sulphate-reducing archaeon Archaeoglobus f ulgi dus: cloning and characterization of the genes and c omparison of t heenzyme with other i ron-sulphu r flavoproteins. Microbiology 140,1273–1284.36. Hipp,W.M.,Pott,A.S.,Thumschmitz,N.,Faath,I.,Dahl,C.&Tru¨per, ... istypical of integral membrane proteins. The polypeptide with anapparent molecular mass of 53 kDa appears as a double band inunboiled samples (lanes A1 and B1).Table 1. N-Terminal sequences of the ... 1899absorption maxima at 420 nm (c band), 530 nm (b band) and 557 nm (a band) are characteristic of cytochrome b.Heme was extracted from the protein with acidic acetone and the pyridine hemochrome spectrum...
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Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
... (2008) A novelplant protein disulfide isomerase family homologous toanimal P5: molecular cloning and characterization as afunctional protein for folding of soybean seed storage proteins. FEBS J ... substrate proteins. Mapping of associa-tion sites of chaperones and PDI family proteins alongindividual substrate polypeptides will be necessary forclarification of the mechanism of action of this protein family. Experimental ... involvement of other PDI family proteins in the folding of the stor-age proteins remains a topic of investigation.In this study, we isolated cDNA clones and genomicsequences encoding soybean group I and...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... identification and characterization of other mem-bers of the CYP719 family, especially in evolutionallyolder plant species, should provide clues to betterunderstand the diversification of the CYP719 family and ... parameters of CYP719A3, the amount of nandinine produced was estima-ted by using the calibration curve of (S)-scoulerine (pmolversus peak area) and the absorption ratio of (S)-scoulerine and (S)-nandinine ... F(2002) Molecular cloning and characterization of coclaurine N-methyltransferase from cultured cells of Coptis japonica. J Biol Chem 277, 830–835.20 Pauli HH & Kutchan TM (1998) Molecular cloning...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... mixture was extracted once with phe-nol ⁄ chloroform and once with chloroform, and RNA wasprecipitated with 2-propanol and dissolved in RNase-freewater. Single-stranded RNAs were allowed to ... roots of the thoracic ganglia and in the subesophagic ganglion of Homarus americanus[15,16]. It is also detected in the retina of the crayfishProcambarus clarkii [17].The cloning and molecular characterization ... & Chan SM (2000) Molecular charac-terization of an additional shrimp hyperglycemic hor-mone: cDNA cloning, gene organization, expression and biological assay of recombinant proteins. FEBSLett...
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