Tài liệu Báo cáo khoa học: Structural and mechanistic aspect

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc

... AnFNR isoalloxazine N5 via S80 [58,62]. In addition, in both enzymes, this residue is critical for proper binding of the nicotinamide to the active centre, CTC stabilization and efficient flavin reduction ... ferredoxin–NADP + reductase. Interactions that stabilize protein– flavin complexes and tailor the midpoint potentials in these proteins, as well as many details of the binding an...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... 273, 22267–22271. 48 Sato Y, Sagami I & Shimizu T (2004) Identiﬁcation of caveolin-1-interacting sites in neuronal nitric-oxide synthase. Molecular mechanism for inhibition of NO for- mation. J Biol Chem ... enzymes will certainly improve our understanding of this essen- tial FMN subdomain interaction. Relationship of K eq A to equilibrium B and to NOS heme reduction At...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... and kinetic isotope effects using PEG-modiﬁed variants and glycoforms of glucose oxidase: the relationship of modiﬁcation of the protein envelope to C–H activation and tunneling. Biochemistry 41, 8747–8758. 72 ... tunnelling Sam Hay, Christopher R. Pudney and Nigel S. Scrutton Manchester Interdisciplinary Biocentre and Faculty of Life Science, University of Manchester, U...

Ngày tải lên : 18/02/2014, 11:20

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... a-helices. b4 in the core domain extends into a b-ribbon with b5, stabilizing loop II. The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel ... Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-17177 Stockholm, Sweden; Department of Anatomy, Physiology and Biochemistry, Swedish University of A...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

... for competitive and noncompetitive inhibitors. For competitive inhibition, the equation is v ¼ V max Æ[S] ⁄ (K m (1 + [I] ⁄ K i ) + [S]), and for noncompetitive inhibition the equation is v ¼ V max Æ[S] ... the kinetic analysis showed posi- tive cooperativity, with an n value of 1.5 ± 0.1. The end-product UTP was a competitive inhibitor against UMP and a noncompetitive inhibitor tow...

Ngày tải lên : 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... extends from the penton base at the vertex positions. Additionally, cementing proteins such as IIIa, VI, VIII and IX help stabilize the capsid, reinforcing the penton–hexon and hexon–hexon interactions. ... exhib- ited impaired endocytosis. Interestingly, in this experi- ment, the ﬁbers were not shed from the virus [19]. These data raise the possibility of coupling of ﬁber relea...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... amplified using gene-specific primers with BamHI and HindIII recogni- tion sites. The resulting PCR fragments were purified and double-digested using BamHI and HindIII, and cloned into pET28a(+) to generate ... genetic and biochemical studies are still required to elucidate the in vivo function of HYD Js ,itis speculative that HYD Js might also be involved in the degradation path...

Ngày tải lên : 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... have important roles in energy metabolism and adenine nucleotide equilibrium. Inhibition of leishmania growth with Ap 5 A, an inhibitor of leishmanial AK2 and its partial reversal by ADP indicate ... the inclusion bodies and refolded in the presence of dithiothreitol, MgSO 4 and KCl showed signiﬁcant enzyme activity. AK activity was linear in terms of both incubation time (up...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

... Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India 2 Department of Biochemistry, Indian Institute of Science, Bangalore, India During stress and the stationary phase of growth, bacterial ... crystallization of the protein The surE gene from S. typhimurium was cloned in an isopropyl thio-b-d-galactoside (IPTG)-inducible vector, overexpressed in E. coli and...

Ngày tải lên : 18/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... the dimer is formed (Fig. 3B). Six of the conserved cysteines are engaged in three intrachain disulﬁde bonds (Cys I- VI, III-VII, V-VIII) stabilizing the cystine knot structure, while two cysteines ... ﬁrst immuno- globulin-like domains (reviewed in [64]). The residues in PDGF-A and -B responsible for receptor binding reside in loop 2, in addition to RKK161 in PDGF- AA and R27 and...

Ngày tải lên : 19/02/2014, 07:20

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