... the peptide is bound in a concentrated mannerat the top of the column, or, if bound to the resinprior to packing the column, the salt gradient concen-trates the peptide on its way out of the ... recom-binant Ab have also been made by several othergroups, but most of these have relied on the generation of Ab fusions. Perhaps the best of these was reported by Lee et al. using a system in ... [41]. The use of thrombin significantlyincreases the cost of the purification, and the require-ment for HPLC increases the length and complexity of the purification procedure. Moreover, as with the studies...