Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 refined models suggest environment-dependent induction of alternative bent ⁄extended resting states doc
... FEBS
Solution properties of full-length integrin
a
IIb
b
3
refined
models suggest environment-dependent induction of
alternative bent
⁄
extended resting states
Camillo Rosano
1
and Mattia Rocco
2
1 Nanobiotecnologie, ... authors.
Refined a
IIb
b
3
models C. Rosano and M. Rocco
3202 FEBS Journal 277 (2010) 3190–3202 ª 2010 The Authors Journal compilation ª 2010...
... mecha-
nism of transphosphorylation, which implies the
A
B
Fig. 3. Stereo diagram of the active center
of HtA. (A) Superposition of the active-site
residues of the 20 conformers of the solu-
tion ... be
considered as an evolutionary intermediate that may
share properties of both protein families, or at least
have acquired some of the properties of the highly
evolved cyto...
... of cytoplasmic composition of anionic li-
pids in membrane binding of K-Ras was evaluated by
studying the effect of ATP depletion, which inhibits
inward movement of PS, with consequent loss of
plasma ... subcellular distribution of K-Ras after dis-
ruption of the electrical properties of the outer and inner leaflets of plasma
membrane and ionic gradients through it. Remov...
... Design and synthesis of a potent
cyclic analogue of the myelin basic protein epitope
MBP72-85: importance of the Ala81 carboxyl group
and of a cyclic conformation for induction of experi-
mental ... a-helicity in a stabilizing solution
environment, and supports the use of DPC-d
38
or
TFE-d
2
[30] as a structure-stabilizing condition for
solution NMR studies of the full-le...
... results suggest that dimerization of
cystatin F is linked to disulfide bond formation, which is
responsible for the loss of inhibitory activity of the pro-
tein. We also noticed that monomerization of ... localization
of cystatin F, its restricted and readily regulated expres-
sion [18], selective and not too potent inhibition of
cathepsins are all in favor of the active role...
... thank Prof. Steve C.
Fry, Institute of Cell and Molecular Biology, Univer-
sity of Edinburgh, Edinburgh, UK, and Dr Albert
Schulte, Ruhr University of Bochum, Germany, for
critical reading of the ... quantities of protein util-
izing the principle of protein-dye binding. Anal Biochem
72, 248–254.
33 Laemmli UK (1970) Cleavage of structural proteins dur-
ing the assembly of the...
... connects the a-helix to strand b
2
of AaHII. This can be
Fig. 5. Solution structure of crotamine. Stereo view of the 26 NMR models superimposed over the backbone atoms of residues 2–39.
1974 G. Nicastro ... residue, located at the end of strand b
1
of Crt,
presents its counterpart in Trp38 similarly located at the end
of strand b
2
of AaHII. Interestingly, chemical modifica...
... one of a few major Myb-interacting proteins. The
moderate basal level of sumoylation was greatly enhanced
by cotransfection of PIASy, an E3 ligase for SUMO-1.
The functional consequence of abolishing ... formed
with low efficiency. This suggests that K527-conjugation
enhances the efficiency of sumoylation at the other site.
A third line of independent evidence for sumoylation of
c...
... contacting
the DNA backbone. The solution structure of the MBD of
MBD1 shows high similarity to that of MeCP2 except for
the C-terminus [18]. At the C-terminus of the MBD, MeCP2
exhibits a one-turn ... complexes
were analyzed by comparison of
1
H-
15
N HSQC spectra of
free MAR-BD with those of MAR-BD titrated with the 15-
mer oligonucleotide duplex at molar ratios of 1 : 1.2...
... One cycle of random SA consisted of
1500 steps of 3 fs at 1000 K followed by 3000 cooling steps
of 1 fs from 1000 K to 100 K. At the end, each structure
was subjected to 1500 steps of conjugate ... analysis of
the model ensemble indicates, a possible hydrogen bond
between the hydrogen of OH9 (H
O
9) and the oxygen of
OH7 (O
H
7) which can contribute to the stabilization of t...