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Tài liệu Finance and Economics Discussion Series Divisions of Research & Statistics and Monetary Affairs Federal Reserve Board, Washington, D C : Interest Rate Risk and Bank Equity Valuations doc

Tài liệu Finance and Economics Discussion Series Divisions of Research & Statistics and Monetary Affairs Federal Reserve Board, Washington, D.C.: Interest Rate Risk and Bank Equity Valuations doc

Tài liệu Finance and Economics Discussion Series Divisions of Research & Statistics and Monetary Affairs Federal Reserve Board, Washington, D.C.: Interest Rate Risk and Bank Equity Valuations doc

... medians of selected bank characteristics for each quintile. The results of this exercise are shown in Table 3.17 Finance and Economics Discussion Series Divisions of Research & Statistics ... in the Finance and Economics Discussion Series (FEDS) are preliminarymaterials circulated to stimulate discussion and critical c omme nt. The analysis and conclusions set forthare those of the ... authors and do not indicate concurrence by other members of the research staff or theBoard of Governors. References in publications to the Finance and Economics Discussion Series (other thanacknowledgement)...
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Tài liệu SWIMMING IN SEWAGE: The Growing Problem of Sewage Pollution and How the Bush Administration Is Putting Our Health and Environment at Risk ppt

Tài liệu SWIMMING IN SEWAGE: The Growing Problem of Sewage Pollution and How the Bush Administration Is Putting Our Health and Environment at Risk ppt

... low-level chemical exposure and chil-dren’s health are difficult to find, “[w]e must steer a middle course between bland indif-ference and blind panic. We cannot afford to pretend that chemicals ... found in sewage, surface, and ground- and drinking-water samples and are recognized as an issue of public health concern.107 The EPA is also conducting research on the presence of illicit drugs ... the dawn of the industrial age, such as sodium hydroxide and sulfuric acid; to more recently engineered compounds such as the toxic plastic additive di(2-ethylhexyl)phthalate (DEHP). Municipalities...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... described above, and thefinal mitochondrial pellets were fixed in NaCl ⁄ Picontaining4% formaldehyde and 2% glutaraldehyde. Mitochondriawere dehydrated through a graded ethanol series and embedded ... CYP2E1Fig. 9. Mitochondrial CYP2E1 activity in wild-type and FDXR-depleted yeast strains. Mitochondrial and microsomal NMDA -d activity in(A) wild-type cells expressing CYP2E1 and (B) FDXR-depleted ... kDa band.This extract also yielded additional antibody-reactivebands of about 28–32 kDa, which probably representdegradation products.Mitochondrial localization of N-terminaltruncated CYP1A1The...
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Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt

... laRecherche contre le Cancer (ARC) (to TG). CDD is arecipient of postdoctoral awards from the InternationalAgency for Research on Cancer, the National CancerCenter and from ARC. Support from ... con-structs indicated on the left, and the introduced Mxi1 isoformswere detected by indirect immunofluorescence using rabbit Mycantibody (Upstate #06-549) as primary antibody and rabbit serumcoupled to ... post-transfection, clones were picked and expanded. Afterinduction with doxycycline (1 lgÆmL)1), the individualclones were tested for their expression of the protein of interest. Flag pull down⁄silver...
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... glucose and hemin chloride (13 lgÆmL)1), and further incubated at30 C for 24 h. Then, the cells were collected and suspen-ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed ... pESC-PSY-CYP93E1 was inoculated in 1 L of SCR-U medium (des-cribed above), and incubated at 30 C for 1 day. Then,50 mL of 40% galactose solution was added (final concen-tration 2%). The cells ... cerevisiaestrain GIL77Two oligo DNAs (5¢-CTTCGTCGACAAGATGTGGAGGTTGAAGATA-3¢ and 5¢-GTCCGCTAGCTCAAGGCAAAGGAACTCTTCT-3¢), corresponding to the N- and C- terminal sequences of b-amyrin synthase from...
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Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx

Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx

... the structure of Tm both freein solution and bound to actin. Thermal unfolding of the Tm coiled-coil can be successfully studied by differ-ent methods such as CD, fluorescence, and DSC.Many ... 599stranded a-helical coiled-coils. J Biol Chem 279 , 21576–21588.37 Lu SM & Hodges RS (2004) Defining the minimum size of a hydrophobic cluster in two-stranded a-helicalcoiled-coils: effects ... Tm5a, and Tm5b, whichare shown in Fig. 5, possessed the double minima at208 and 222 nm characteristic of the correctly folded,fully a-helical structure of Tm. The CD spectra of Tm5a and Tm5b...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... argininesubstitutionsEach of the RTA variants described above were reassoci-ated with RTB and the cytotoxicity of each of the resultantricin variants was compared to wild-type ricin. RicinR213A and ricin R21 3D ... fungi and bacteria [1]. Ricinconsists of a catalytic A chain (RTA) joined by a singledisulphide bond to a lectin B chain (RTB) that facilitatesboth cell surface binding and entry of the catalyticallyactive ... Symbols indicate the mean of four replicate samples,error bars represent the SD. (A) ricin, d, ricin R213A, j;andricinR21 3D, m.(B)ricin ,d; ricin R258A, j, and ricin R25 8D, m; (C) ricin, d; ricin R48A,...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... primers: 5¢-GGGCACTCAGCCAGGGGACATCCTGCCCAA-3¢ as forward and 5¢-GATACAAGTTTGCACACCTTTGCACTTCTG-3¢ asreverse [58]. The PCR amplification was performed in atotal volume of 50 lL reaction mix containing ... Iron-induced upregulation of cell surface CD 1d is accompanied by an increase in total protein and mRNA transcripts. HepG2cells were cultured as indicated in legend of Fig. 1 and cells processed for ... asCD 1d. Materials and methodsCells and culture conditionsThe hepatocellular carcinoma cell line HepG2 was pur-chased from the European Collection of Cell Cultures(ECACC, Wiltshire, UK) and...
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... E,Kalbacher H, Nordheim A & Driessen C (2004) HumanB lymphoblastoid cells contain distinct patterns of cathepsin activity in endocytic compartments and regu-late MHC class II transport in a cathepsin ... Immunomodula-tory properties have been demonstrated for anothertype II cystatin, cystatin C. The process of dendriticcell maturation leads to a reduced level and distinctintracellular distribution ... significance of the inhibition data, the intracellular localization of cystatin F and its potential targets, cathepsins B, H, L, S, C and K,were studied by confocal microscopy in U937 promonocyte cells....
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... 5¢-CTTTGCGATGACTTACAACTTCTACGGCGG-3¢,5¢-GTAGATATTGACTGGATGTTCCCTGGTGGCGGCG-3¢ and 5¢-GATATTGACTGGCAATTCCCTGGTGGCGGC-3¢, and the reverse oligo-nucleotide sequences were 5¢-CAGCCGCCGTAGAAGTTGTAAGTCATCGCAAAG-3¢,5¢-CGCCGCCACCAGGGAACATCCAGTCAATATCTAC-3, ... reactionwas quenched after the indicated reaction times at 20 C by theaddition of acetic acid to 10% and applied to calibrated HPLC-MS.For each substrate, the calculated concentrations of ... order of (Glc-NAc)6> (GlcNAc)4> (GlcNAc)3, and showed no activity towards (Glc-NAc)2 and pNP-GlcNAc. This suggested that the binding site of chitinaseA was probably composed of...
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