... InsP6gradually into InsP5, InsP4, and the final product – Ins (2, 4,6)P3 and Ins(1,3,5)P3– via two alternative pathways [14].Bacterial BPPs containing two tandemly repeateddomains (dual domains) ... they can increase the amount of available phosphate by interacting together. Additionally,fusing PhyH-DI to a single-domain phytase appears to be an efficient way to improve the activity of the ... Trans1-T1 (TransGen, Beijing, China) and pGEM-TEasy (Promega, Madison, WI, USA) were used for genecloning and sequencing, respectively. E. coli BL21 (DE3)(TaKaRa, Ostu, Japan) and pET -22 b(+)...