... volumes of 5M urea solution and then run on SDS ⁄ PAGE. Lanes a and d, inthe absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in ... Complex forma-tion was detected by the bands of the TnI–TnC complex (arrowheads) and weakening of the free TnC bands. Free RTnI, RTnI1)116,RTnI96)181, ATnI-19K, ATnI130)252, and ATnI232)292did ... presence (lanes m–q) of equimolar amounts of Akazara scallop TnC. Lanes h and m, ATnI-52K; lanesi and n, ATnI-19K; lanes j and o, ATnI1)128; lanes k and p, ATnI130)252; lanes l and q, ATnI232)292;...