... their cognate chaperones and are injected through the translocon pore and into the target
cytoplasm. IM, inner membrane; MO, outer membrane.
P J. Matteı
¨
et al. Membrane targeting and pore formation ... Journal 278 (2011) 414–426 ª 2010 The Authors Journal compilation ª 2010 FEBS
REVIEW ARTICLE
Membrane targeting and pore formation by the type III...
... this type exhibit the predicted
amount of binding only if the heterodimer is tethered
by the wild -type partner. By contrast, we found that, if
the heterodimer is tethered to the resin by the mutant
partner, ... on the function
of the contralateral binding site. The mutant site does
not prevent the wild -type site from binding IGF-II and
pairing with a wild -ty...
... detect and discriminate between thou-
sands of different odorant molecules is governed by the diverse array of
olfactory receptors expressed by olfactory sensory neurons in the nasal
epithelium. ... further depolarizing the cell. How these diverse
signaling molecules find each other in the complex
and densely-packed environment of the cell, avoiding
cross-talk with other sign...
... between
J(0) and J(x
N
) and between J(0) and J(0.87x
H
) for the
TTF-1 HD were then examined as proposed by Lefe
`
-
vre et al. [21]. The fit was obtained by linear
regression, and only the corresponding ... interruption of the geometry
of the recognition helix for either Antp or the TTF-1
HD, the anomalous amide exchange pattern and the
NOE connectivity data of...
... 2008 The Authors Journal compilation ª 2008 FEBS 4391
Zn-binding motif and the two disulfide bonds respec-
tively connecting the a4-helix to the b4 strand and the
b9 strand to the b10 strand at the ... concentration
of 3.4 lm.
Thermophilicity and thermostability
Thermophilicity was evaluated in the temperature range
40–90 °C by measuring the polymerase activity of th...
... [49], and a
CHO-derived cell line [50]. However, the site at which
the PI3K signaling feeds into the Erk activating path-
way varies in these systems: at the Ras, Raf or MEK
activation level. In the ... and required for the for-
mation of epithelia in the embryo and the maintenance
of the polarized epithelial structure in the adult [2]. As
a single-span transmembrane...
... in the association of the translational
complex with the microsomal membrane [13,14]. Thus,
the N-terminal hydrophobic sequences of CYPs are
important for their targeting to and retention in the
ER ... targeting
of CYPs to the ER and mitochondria, Alzheimer’s
amyloid precursor protein to the plasma membrane
and mitochondria, and translocation of the cytosolic
g...
... within the cyto-
plasm driven by the plasma membrane potential and then
be further accumulated within the mitochondria due to the
mitochondrial membrane potential [11]. From the known
plasma and ... driven by
theplasmamembranepotential(Dw
p
) followed by the further accu-
mulation of mitoDC-81 into mitochondria driven by the mitoch-
ondrial membrane potential (...
... lactoperoxidase-glucose oxidase. Membrane proteins
were then isolated by biotinylation and streptavidin/agarose
extraction, analysed by SDS/PAGE and autoradiographed.
The 67-kDa LBP, together with other membrane proteins
were ... in both the trypsin
released supernatant and the cell pellet. In other words, both
the 27-kDa fragment of LBP released by trypsin as well as...
... of the heme groups of the enzyme by
various compounds was tested to identify the physiological
electron acceptor of the enzyme. The enzyme was not
oxidized by the heterodisulfide of coenzyme M and
coenzyme ... with the other subunits
of the enzyme complex.
From the primary structure, the b -type cytochrome-like
protein AF501 and subunit AF500 are clearly predicted...