Tài liệu Báo cáo khoa học: How to remain nonfolded and pliable: the linkers in modular a-amylases as a case study ppt

... How to remain nonfolded and pliable: the linkers in modular a- amylases as a case study Georges Feller1, Dominique Dehareng1 and Jean-Luc Da Lage21 Center for Protein Engineering, University ... bondhydrolyzed has the theoretical capacity to disrupt a hydrophobic interaction in the linker, inducing orfavoring its extension. As the catalytic constant kcatofanimal-type a- amylases is in the range ... the participatingTable 1. Amino acid frequencies (%) in a- amylase linkers, in a setof globular proteins, in the Swiss-Prot databank and in intrinsicallyunstructured proteins.Aminoacid Linkers a GlobularproteinsbSwiss-ProtcIntrinsicallyunstructuredproteinsbAla...

Tài liệu Báo cáo khoa học: "HOW TO MISREAD A DICTIONARY" pot

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Tài liệu Báo cáo khoa học: "Learning to Find Translations and Transliterations on the Web" doc

... snippets, and generate features for each tokens in the same way as done in the training phase. We then use the trained model to tag the snippets, and extract translation candidates by identifying ... requiring minimal human intervention to prepare the training data. 3 Method To find translations for a given term on the Web, a promising approach is automatically learning to extract phrasal ... additional training data for a machine translation system, as described in Lin et al. (2008). 2 Related Work Phrase translation and transliteration is important for cross-language tasks. For example,...

Tài liệu Báo cáo khoa học: Globin gene family evolution and functional diversiﬁcation in annelids ppt

... mutabilis NassaMb P31331Scapharca inaequivalvis ScaHb1 Q26505Anadara trapezia HBIaAna P14395HBIIAna P14394Barbatia lima BarbHBD Q17157Biomphalaria glabrata HBD2Biom and HBD3BiomQ683R3Artemia ... 489 CAGgt aaag ctaac ttgc ag GTAl. pompejana Hb Intron 1 B12.2 135 GGAgt aagt ctaac accc ag GTIntra Intron 2 G7.0 241 GCTgt aagt ctaac tttc ag GAAr. marina Mb Intron 1 B12.2 301 CTTgt aagt ... evolutionaryconvergence between proteins exhibiting structuralsimilarity.We have shown that R. pachyptila and Ar. marinaextracellular globins (A2 and B2), Ar. marina Mb and Al. pompejana and G....

Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt

... To amplify the DNA frag-ments containing a complete x-5 gliadin gene, oligonucleo-tides, 5 ¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢ and 5¢-CGTTACATTATGCTCCATTGACTAACAACGATG-3¢, were constructed based ... frozenleaves by the Isoplant DNA extraction Kit (Takara Bio Inc.,Shiga, Japan). PCR was performed using KOD DNA polym-erase (Toyobo, Osaka, Japan) and DNA AMPLIFIERMIR-D40 (Sanyo, Osaka, Japan). To ... sequencing kit and the ABI 3100 DNA sequencer(Applied Biosystems, Foster City, CA, USA).Expression and puriﬁcation of recombinantproteinSense (5¢-ATTTCATATGCAACAACAATTCCCCCAGCAACAATCA-3¢) and antisense...

Tài liệu Báo cáo khoa học: How disorder inﬂuences order and vice versa – mutual effects in fusion proteins containing an intrinsically disordered and a globular protein docx

... an N-terminalhexahistidine tag was obtained by PCR using the pET2 1a ⁄ PNT-H6plasmid [30] as the template. The forwardprimer (5¢-TACCGTTAACATCGATATGCATCATCATCATCATCATGC-3¢) was designed to ... hexahistidinetag fused to its N-terminus was obtained by PCR from the plasmid pET21 ⁄ SIC1 [32] with a forward primer (5¢-TACCTGGCCAATGAATATGCATCATCATCATCATCATACTCCGTCGACCCCACC-3¢) designed to ... PNT. The struc-tural disorder and ﬂexibility typical of isolated NTAILis maintained and appears to increase in the fusion,resulting in a Stokes radius that is even higher thanexpected from the...

Tài liệu Báo cáo khoa học: How does hepatitis C virus enter cells? pptx

... to the N-terminalprotease domain, the carboxy-terminal domain of NS3consists of an RNA helicase and NTPase activity.NS 4A serves as a cofactor for NS3. The functions ofNS4B and NS 5A are largely ... of clinical HCV isolates is not as clear. Antibodiesagainst CD81 or recombinant CD81 had no or only a marginal effect on the binding and internalization (as measured by the a- sarcin assay) of ... DC-SIGN and the asialoglycoproteinreceptor (ASGPR). Heparan sulfate, a glycosaminogly-can in the plasma membrane of many cells, also binds to recombinant E2 with high afﬁnity [58] and blocksbinding...

Tài liệu Báo cáo khoa học: Strategies to cover microbial proteomes ppt

... spatial and temporal resolution for imaging neocortical functions in the liv-ing brain, and has paved the way for a new era in the functionalimaging of cortical dynamics. It has facilitated the ... vaccine candidate was successful as vaccine in animalmodels and is now in a clinical study. A proteome 2-DE databasewas established (http://www.mpiib-berlin.mpg.de/2D-PAGE),which contains data of ... leads to activation of the receptor tyrosine kinase and initiation of retrograde transport from the ﬁlopodia to the cellbody. According to a FRET assay based on QDs as donors,endocytosis of the...

Tài liệu Báo cáo khoa học: An engineered disulﬁde bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... calculated from the intensity of the protein band.Autoproteolysis in sample solutions containing <20lgÆmL)1protein was followed by determining the residualactivity towards casein or the ... 300–319 amino acid residues and are organized into twodomains. They have one catalytic zinc ion, and between two and four stabilizing calcium ions. The X-ray structures of thermolysin [2] and the ... play a cru-cial role [24]. To date, almost all stability investigations of the neutral protease from B. stearothermophilus and itsvariants have been based on thermoinactivation meas-urements assuming...

Tài liệu Báo cáo khoa học: "Learning to Translate with Multiple Objectives" doc

... vector w, takes in a foreign sen-tence f and returns a translated hypothesis h. The argmax operates in vector space and our goal is to ﬁnd w leading to hypotheses on the Pareto Frontier. In the ... dominating point.ilar to many MT optimization methods. The maindifference is that rather than trying to maximize a single metric, we maximize the number of paretopoints, in order to expand the ... Association for Computational LinguisticsLearning to Translate with Multiple ObjectivesKevin Duh∗Katsuhito Sudoh Xianchao Wu Hajime Tsukada Masaaki NagataNTT Communication Science Laboratories2-4...

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