... Analysis of data wasperformed withORIGIN6.1 andSIGMA PLOT4.2 software. A direct plot of activity vs. [GTP] gave in all cases a sigmoidalcurve indicating cooperative substrate binding. In order ... Biochemical analysis of a dimerization domain mutation in RetGC-1 associated withdominant cone-rod dystrophy. Proc. Natl Acad. Sci. USA 96,9039–9044.16. Duda, T., Venkataraman, V., Jankowska, A. , ... toanalyze data of a sigmoidal dependence in a linearLineweaver–Burk plot [41], we determined a Hill coefficientn from a fit of the direct plot. Values of Vmaxand Kmwerethen determined from...